PMC

Fig. 22D-IR spectra and kinetics of each labeled peptide in the presence of DOPC vesicles prepared with cholesterol. 2D IR snapshots of L12A13-labeled hIAPP aggregation at 5 minutes (A), 150 minutes (B), and 350 minutes (C). (D) The diagonal of 2D IR spectra in the isotope labeled region of L12A13-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 2 states, shown in red and blue in (E). (F) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (E). 2D IR snapshots of L16V17-labeled hIAPP aggregation at 5 minutes (G), 150 minutes (H), and 350 minutes (I). (J) The diagonal of 2D IR spectra in the isotope labeled region of L16V17-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 2 states, shown as a red and blue trace in (K). (L) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (K). 2D IR snapshots of G24A25-labeled hIAPP aggregation at 5 minutes (M), 150 minutes (N), and 350 minutes (O). (P) The diagonal of 2D IR spectra in the isotope labeled region of G24A25-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 3 states, shown in red, green, and blue in (Q). (R) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (Q). 2D IR snapshots of V32G33-labeled hIAPP aggregation at 5 minutes (S), 125 minutes (T), and 350 minutes (U). (V) The diagonal of 2D IR spectra in the isotope labeled region of V32G33-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time dependent diagonal trace reveals 2 states, shown as red and blue traces in (W). (X) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (W).

Fig. 12D-IR spectra and kinetics of labeled hIAPP in Tris buffer. 2D IR snapshots of L12A13-labeled hIAPP aggregation at 5 minutes (A), 150 minutes (B), and 500 minutes (C). (D) The diagonal of 2D IR spectra in the isotope labeled region of L12A13-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 3 state, shown in red, green, and blue in (E). (F) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (E). 2D IR snapshots of L16V17-labeled hIAPP aggregation at 5 minutes (G), 250 minutes (H), and 500 minutes (I). (J) The diagonal of 2D IR spectra in the isotope labeled region of L16V17-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 2 states, shown as a red and blue trace in (K). (L) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (K). 2D IR snapshots of G24A25-labeled hIAPP aggregation at 5 minutes (M), 125 minutes (N), and 350 minutes (O). (P) The diagonal of 2D IR spectra in the isotope labeled region of G24A25-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time-dependent diagonal trace reveals 3 states, shown in red, green, and blue in (Q). (R) displays kinetics plots of the intensities of the matrix-decomposition traces shown in (Q). 2D IR snapshots of V32G33-labeled hIAPP aggregation at 5 minutes (S), 100 minutes (T), and 500 minutes (U). (V) The diagonal of 2D IR spectra in the isotope labeled region of V32G33-labeled hIAPP at 5 time points during aggregation. Matrix decomposition of the time dependent diagonal trace reveals 2 states, shown as red and blue traces in (W). (X) Displays kinetics plots of the intensities of the matrix-decomposition traces shown in (W). Data presented in A–L was previously published by Maj et al. and is reproduced here for ease of comparison.

Figure 1

Figure 5